In vitro effect of aqueous extracts from different plants on the development of the exogenous stages of gastrointestinal strongyles in sheep

Mileydy Puerto Abreu

ABSTRACT
In order to evaluate in vitro the antiparasitic properties of Moringa oleifera, Gliricidia sepium and Dichrostachys cinerea on exogenous stages of the biological cycle of gastrointestinal nematodes of sheep, the effect of aqueous extracts on the inhibition of egg hatching and migration of third-stage larvae was evaluated. For such purpose, aqueous extracts were prepared for each plant in three concentrations (50, 25 and 12,5 mg/mL), and their effect on egg hatching and larvae migration was determined, compared with positive and negative control. The three plants inhibited the egg hatching capacity with higher (p < 0,05) hatching inhibition rates for D. cinerea and M. oleifera. The Probit analysis showed that the mean lethal dose for each species was 60.92, 30.70 and 9.41 mg/mL for M. oleifera, G. sepium and D. cinerea, respectively. The three plants showed effects on the larvae migration compared with the controls, with the highest migration inhibition rates through a 20-µm sieve (p < 0,05) for M. oleifera and G. sepium, showing values over 97 %. The in vitro results suggest that the three plants have antiparasitic properties that could be evaluated in later in vivo studies.